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Aerobic ammonia and nitrite oxidation reactions are fundamental biogeochemical reactions contributing to the global nitrogen cycle. Although aerobic nitrite oxidation yields 4.8‐folds less Gibbs free energy (∆Gr) than aerobic ammonia oxidation in the NH4+‐feeding marine recirculating trickling biofilter reactors operated in the present study, nitrite‐oxidizing and not ammonia‐oxidizing Nitrospira...
In situ detection of microorganisms by fluorescence in situ hybridization (FISH) is a powerful tool for environmental microbiology, but analyses can be hampered by low rRNA content in target organisms, especially in oligotrophic environments. Here, we present a non‐enzymatic, hybridization chain reaction (HCR)‐based signal amplified in situ whole‐cell detection technique (in situ DNA‐HCR). The components...
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