Search results for: Bishwajit Kundu

Items from 1 to 20 out of 28 results

article

Evolutionarily conserved heat shock protein, HtpX, as an adjunct target against antibiotic‐resistant Neisseria gonorrhoeae

Shubham Vashishtha, Sheetal Thakur, Jasdeep Singh, Sujata Adhana, more

Journal of Cellular Biochemistry > 124 > 10 > 1516 - 1529

The emergence of multiple drug resistance and extreme drug resistance pathogens necessitates the continuous evaluation of the pathogenic genome to identify conserved molecular targets and their respective inhibitors. In this study, we mapped the global mutational landscape of Neisseria gonorrhoeae (an intracellular pathogen notoriously known to cause the sexually transmitted disease gonorrhoea). We...

article

Antimicrobial‐resistant Neisseria gonorrhoeae can be targeted using inhibitors against evolutionary conservedl‐asparaginase

Shubham Vashishtha, Jasdeep Singh, Bishwajit Kundu

Journal of Cellular Biochemistry > 123 > 7 > 1171 - 1182

The emergence of multiple drug‐resistant “super gonorrhoea” complicates the management and treatment of Neisseria gonorrhoeae infections due to the progressive accumulation of mutations in the biological targets of frontline antimicrobials. Continuous evaluation and reporting of newer molecular targets and their inhibitors are necessary. Here, we present l‐asparaginase of N. gonorrhoeae (NgA) as a...

article

Most Variable Genes and Transcription Factors in Acute Lymphoblastic Leukemia Patients

Anil Kumar Tomar, Rahul Agarwal, Bishwajit Kundu

Interdisciplinary Sciences: Computational Life Sciences > 2019 > 11 > 4 > 668-678

Acute lymphoblastic leukemia (ALL) is a hematologic tumor caused by cell cycle aberrations due to accumulating genetic disturbances in the expression of transcription factors (TFs), signaling oncogenes and tumor suppressors. Though survival rate in childhood ALL patients is increased up to 80% with recent medical advances, treatment of adults and childhood relapse cases still remains challenging....

article

Identification and validation of l‐asparaginase as a potential metabolic target against Mycobacterium tuberculosis

Arti Kataria, Jasdeep Singh, Bishwajit Kundu

Journal of Cellular Biochemistry > 120 > 1 > 143 - 154

Multidrug‐resistant Mycobacterium tuberculosis (Mtb) has emerged as a major health challenge, necessitating the search for new molecular targets. A secretory amidohydrolase, l‐asparaginase of Mtb (MtA), originally implicated in nitrogen assimilation and neutralization of acidic microenvironment inside human alveolar macrophages, has been proposed as a crucial metabolic enzyme. To investigate whether...

article

Endoplasmic Reticulum Stress Induces Myostatin High Molecular Weight Aggregates and Impairs Mature Myostatin Secretion

Rishibha Sachdev, Karin Kappes-Horn, Lydia Paulsen, Yvonne Duernberger, more

Molecular Neurobiology > 2018 > 55 > 11 > 8355-8373

Sporadic inclusion body myositis (sIBM) is the most prevalent acquired muscle disorder in the elderly with no defined etiology or effective therapy. Endoplasmic reticulum stress and deposition of myostatin, a secreted negative regulator of muscle growth, have been implicated in disease pathology. The myostatin signaling pathway has emerged as a major target for symptomatic treatment of muscle atrophy...

article

Heterologous expression of an engineered protein domain acts as chaperone and enhances thermotolerance of Escherichia coli

Rajender Jena, Dushyant K. Garg, Lipsa Choudhury, Akanksha Saini, more

International Journal of Biological Macromolecules > 2018 > 107 > PB > 2086-2093

Heat shock proteins (HSPs) are known to confer protection to the stressed cells by rescuing vital host cell proteins. In the present study we have demonstrated that heterologous expression of N-terminal domain of hyperthermophilic L-asparaginase (NPfA) confers thermotolerance to E. coli. The recombinant expression of NPfA enabled E. coli to demonstrate typical growth behavior at 52°C and survive a...

article

Interplay between CedA, rpoB and double stranded DNA: A step towards understanding CedA mediated cell division in E. coli

Pankaj Sharma, Anil Kumar Tomar, Bishwajit Kundu

International Journal of Biological Macromolecules > 2018 > 107 > PB > 2026-2033

Cell division is compromised in DnaAcos mutant E. coli cells due to chromosome over-replication. In these cells, CedA acts as a regulatory protein and initiates cell division by a hitherto unknown mechanism. CedA, a double stranded DNA binding protein, interacts with various subunits of RNA polymerase complex, including rpoB. To reveal how this concert between CedA, rpoB and DNA brings about cell...

article

Identification of functional interactome of a key cell division regulatory protein CedA of E.coli

Pankaj Sharma, Anil Kumar Tomar, Bishwajit Kundu

International Journal of Biological Macromolecules > 2018 > 106 > C > 763-767

Cell division is compromised in DnaAcos mutant Escherichia coli cells that results in filamentous cell morphology. This is countered by over-expression of CedA protein that induces cytokinesis and thus, regular cell morphology is regained; however via an unknown mechanism. To understand the process systematically, exact role of CedA should be deciphered. Protein interactions are crucial for functional...

article

Biophysical insight into the heparin‐peptide interaction and its modulation by a small molecule

Neha Tiwari, Ankit Srivastava, Bishwajit Kundu, Manoj Munde

Journal of Molecular Recognition > 31 > 1 > n/a - n/a

The heparin‐protein interaction plays a vital role in numerous physiological and pathological processes. Not only is the binding mechanism of these interactions poorly understood, studies concerning their therapeutic targeting are also limited. Here, we have studied the interaction of the heparin interacting peptide (HIP) from Tat (which plays important role in HIV infections) with heparin. Isothermal...

article

L-Asparaginase of Leishmania donovani: Metabolic target and its role in Amphotericin B resistance

Jasdeep Singh, Mohd Imran Khan, Shiv Pratap Singh Yadav, Ankit Srivastava, more

International Journal for Parasitology: Drugs and Drug Resistance > 2017 > 7 > 3 > 337-349

Emergence of Amphotericin B (AmB) resistant Leishmania donovani has posed major therapeutic challenge against the parasite. Consequently, combination therapy aimed at multiple molecular targets, based on proteome wise network analysis has been recommended. In this regard we had earlier identified and proposed L-asparaginase of Leishmania donovani (LdAI) as a crucial metabolic target. Here we report...

article

Hyperthermophilic l-asparaginase bypasses monomeric intermediates during folding to retain cooperativity and avoid amyloid assembly

Dushyant K. Garg, Bishwajit Kundu

Archives of Biochemistry and Biophysics > 2017 > 622 > C > 36-46

In obligate dimeric proteins of hyperthermophilic origin the question whether the native dimer is obtained by association of folded monomers or through concomitant folding and assembly of subunits has intrigued researchers. To find an answer we studied the folding of a dimeric enzyme l-asparaginase from Pyrococcus furiosus (PfA) for which we reported earlier that it unfolds cooperatively without populating...

article

Polyphenols in combination with β-cyclodextrin can inhibit and disaggregate α-synuclein amyloids under cell mimicking conditions: A promising therapeutic alternative

Saurabh Gautam, Sandip Karmakar, Radhika Batra, Pankaj Sharma, more

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics > 2017 > 1865 > 5 > 589-603

Parkinson's disease is characterized by the presence of insoluble and neurotoxic aggregates (amyloid fibrils) of an intrinsically disordered protein α-synuclein. In this study we have examined the effects of four naturally occurring polyphenols in combination with β-cyclodextrin (β-CD) on the aggregation of α-synuclein in the presence of macromolecular crowding agents. Our results reveal that even...

article

Antifungal activities of selected essential oils against Fusarium oxysporum f. sp. lycopersici 1322, with emphasis on Syzygium aromaticum essential oil

Abhishek Sharma, Sasireka Rajendran, Ankit Srivastava, Satyawati Sharma, more

Journal of Bioscience and Bioengineering > 2017 > 123 > 3 > 308-313

The antifungal effects of four essential oils viz., clove (Syzygium aromaticum), lemongrass (Cymbopogon citratus), mint (Mentha × piperita) and eucalyptus (Eucalyptus globulus) were evaluated against wilt causing fungus, Fusarium oxysporum f. sp. lycopersici 1322. The inhibitory effect of oils showed dose-dependent activity on the tested fungus. Most active being the clove oil, exhibiting complete...

article

Clues for divergent, polymorphic amyloidogenesis through dissection of amyloid forming steps of bovine carbonic anhydrase and its critical amyloid forming stretch

Dushyant Kumar Garg, Bishwajit Kundu

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics > 2016 > 1864 > 7 > 794-804

Certain amino acid stretches are considered ‘critical’ to trigger amyloidogenesis in a protein. Synthetic peptides corresponding to these stretches are often used as experimental mimics for studying the amyloidogenesis of their parent protein. Here we provide evidence that such simple extrapolation is misleading. We scrutinized each step of amyloid progression of full length bovine carbonic anhydrase...

article

Structural and functional insights into an archaeal l‐asparaginase obtained through the linker‐less assembly of constituent domains. Corrigendum

Rachana Tomar, Pankaj Sharma, Ankit Srivastava, Saurabh Bansal, more

Acta Crystallographica Section D > 72 > 1 > 180 - 180

A correction is made to Fig. 7 in the article by Tomar et al. [(2014). Acta Cryst. D70, 3187–3197].

article

Stable self-assembled nanostructured hen egg white lysozyme exhibits strong anti-proliferative activity against breast cancer cells

Sailendra Mahanta, Subhankar Paul, Ankit Srivastava, Ashutosh Pastor, more

Colloids and Surfaces B: Biointerfaces > 2015 > 130 > C > 237-245

Chemotherapy side effects have long been a matter of great concern. Here we describe a structurally stable self-assembled nanostructured lysozyme (snLYZ) synthesized using a simple desolvation technique that exhibited anticancer activity, as well as excellent hemocompatibility. Field emission scanning electron microscopy; atomic force microscopy and dynamic particle size analyzer were used for analyzing...

article

Curcumin binds to the pre-fibrillar aggregates of Cu/Zn superoxide dismutase (SOD1) and alters its amyloidogenic pathway resulting in reduced cytotoxicity

Nidhi K. Bhatia, Ankit Srivastava, Nidhi Katyal, Nidhi Jain, more

BBA - Proteins and Proteomics > 2015 > 1854 > 5 > 426-436

Amyotrophic Lateral Sclerosis (ALS) is a fatal neurodegenerative disease that affects motor neurons. Unfortunately, effective therapeutics against this disease is still not available. Almost 20% of familial ALS (fALS) is suggested to be associated with pathological deposition of superoxide dismutase (SOD1). Evidences suggest that SOD1-containing pathological inclusions in ALS exhibit amyloid like...

article

Domains of Pyrococcus furiosus l-asparaginase fold sequentially and assemble through strong intersubunit associative forces

Dushyant K. Garg, Rachana Tomar, Reema R. Dhoke, Ankit Srivastava, more

Extremophiles > 2015 > 19 > 3 > 681-691

Here, we report the folding and assembly of a Pyrococcus furiosus -derived protein, l-asparaginase (PfA). PfA functions as a homodimer, with each monomer made of distinct N- and C-terminal domains. The purified individual domains as well as single Trp mutant of each domain were subjected to chemical denaturation/renaturation and probed by combination of spectroscopic, chromatographic,...

article

Structural and functional insights into an archaeal L‐asparaginase obtained through the linker‐less assembly of constituent domains

Rachana Tomar, Pankaj Sharma, Ankit Srivastava, Saurabh Bansal, more

Acta Crystallographica Section D > 70 > 12 > 3187 - 3197

Covalent linkers bridging the domains of multidomain proteins are considered to be crucial for assembly and function. In this report, an exception in which the linker of a two‐domain dimeric L‐asparaginase from Pyrococcus furiosus (PfA) was found to be dispensable is presented. Domains of this enzyme assembled without the linker into a conjoined tetrameric form that exhibited higher activity than...

article

Thermodynamic Stabilization of the Folded Domain of Prion Protein Inhibits Prion Infection in Vivo

Qingzhong Kong, Jeffrey L. Mills, Bishwajit Kundu, Xinyi Li, more

Cell Reports > 2013 > 4 > 2 > 248-254

Prion diseases, or transmissible spongiform encephalopathies (TSEs), are associated with the conformational conversion of the cellular prion protein, PrP ^C , into a protease-resistant form, PrP ^Sc . Here, we show that mutation-induced thermodynamic stabilization of the folded, α-helical domain of PrP ^C has a dramatic inhibitory effect on the conformational conversion of prion...

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Keywords

L‐ASPARAGINASE (5)
AMYLOID (3)
PROTEIN AGGREGATION (3)
AGGREGATION (2)
ANTIMICROBIAL RESISTANCE (2)
BOVINE CARBONIC ANHYDRASE (2)
CELL VIABILITY ASSAY (2)
DOMAIN (2)
FOLDING (2)
L-ASPARAGINASE (2)
LINKER‐LESS ASSEMBLY (2)
MICROSCOPY (2)
MOLECULAR DYNAMIC SIMULATIONS (2)
MULTIMERIC (2)
NEISSERIA GONORRHOEAE (2)
PYROCOCCUS FURIOSUS (2)
VIRTUAL SCREENING (2)
AGGREGATE STRUCTURES (1)
ALTERNATE STRUCTURE (1)
AMPHOTERICIN B RESISTANCE (1)
AMYLOID FIBRIL (1)
AMYLOID INHIBITION AND DISAGGREGATION (1)
AMYLOID POLYMORPHISM (1)
AMYLOID PRECURSOR PROTEIN (1)
AMYLOIDOGENESIS (1)
AMYLOIDS (1)
AMYOTROPHIC LATERAL SCLEROSIS (1)
ATROPHY (1)
BINDING THERMODYNAMICS (1)
BIOPHYSICAL TECHNIQUES (1)
BREAST CANCER (1)
CEDA (1)
CEDA-BINDING PROTEINS (1)
CELL DIVISION (1)
CHAPERONE (1)
CHAPERONINS (1)
CHROMOSOMAL DNA OVER-REPLICATION (1)
CHROMOSOME OVER-REPLICATION (1)
CIRCULAR DICHROISM (1)
CO-OPERATIVITY (1)
COOPERATIVITY (1)
COPPER (1)
CORRIGENDUM (1)
CRITICAL AMYLOID STRETCH (1)
CRITICAL REGIONS (1)
CRYSTALLIN (1)
CURCUMIN (1)
CYTOPLASMIC GRANULES (1)
E. COLI. (1)
ER STRESS (1)
ESSENTIAL OILS (1)
FLUORESCENCE SPECTROSCOPY (1)
FTIR (1)
FUSARIUM OXYSPORUM F. SP. LYCOPERSICI (1)
GENE EXPRESSION (1)
HEAT SHOCK PROTEINS (1)
HEAT-SHOCK DOMAIN (1)
HEPARIN (1)
HOMOLOGY MODELING (1)
HYDROPHOBIC INTERACTIONS (1)
HYDROPHOBICITY (1)
HYPERTHERMOPHILIC PROTEIN (1)
INHIBITOR (1)
INHIBITORS (1)
KEGG PATHWAYS (1)
KINETIC STABILITY (1)
LEISHMANIA DONOVANI (1)
LENS CRYSTALLINS (1)
LEUKEMIA (1)
LOCAL CONTACTS (1)
METABOLIC TARGET (1)
MINIMUM INHIBITORY CONCENTRATION (1)
MOLECULAR CHAPERONE (1)
MOLECULAR DOCKING (1)
MOLECULAR DYNAMICS SIMULATIONS (1)
MOST VARIABLE GENES (1)
MTT ASSAY (1)
MYCOBACTERIUM (1)
MYOSTATIN (1)
NEURODEGENERATION (1)
PARKINSON'S DISEASE (1)
PEPTIDE (1)
PEPTIDE SCANNING (1)
POLYPHENOLS (1)
PRIONS (1)
PROTEIN FOLDING (1)
PROTEIN MISFOLDING (1)
PROTEIN-DNA INTERACTION (1)
PROTEIN‐LIGAND INTERACTIONS (1)
PULL DOWN ASSAY (1)
RECURRENCE QUANTIFICATION ANALYSIS (1)
RECURRENT QUANTIFICATION ANALYSIS (1)
RPOB (1)
SCRAPIE (1)
SECONDARY NUCLEATION (1)
SELF-ASSEMBLED NANOSTRUCTURED LYSOZYME (1)
SEQUENTIAL FOLDING (1)
SPORADIC INCLUSION BODY MYOSITIS (1)
STABILITY (1)
SUBTYPE CLASSIFICATION (1)
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Elsevier (18)
Wiley (7)
Springer (3)

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Search results for: Bishwajit Kundu

Evolutionarily conserved heat shock protein, HtpX, as an adjunct target against antibiotic‐resistant Neisseria gonorrhoeae

Antimicrobial‐resistant Neisseria gonorrhoeae can be targeted using inhibitors against evolutionary conservedl‐asparaginase

Most Variable Genes and Transcription Factors in Acute Lymphoblastic Leukemia Patients

Identification and validation of l‐asparaginase as a potential metabolic target against Mycobacterium tuberculosis

Endoplasmic Reticulum Stress Induces Myostatin High Molecular Weight Aggregates and Impairs Mature Myostatin Secretion

Heterologous expression of an engineered protein domain acts as chaperone and enhances thermotolerance of Escherichia coli

Interplay between CedA, rpoB and double stranded DNA: A step towards understanding CedA mediated cell division in E. coli

Identification of functional interactome of a key cell division regulatory protein CedA of E.coli

Biophysical insight into the heparin‐peptide interaction and its modulation by a small molecule

L-Asparaginase of Leishmania donovani: Metabolic target and its role in Amphotericin B resistance

Hyperthermophilic l-asparaginase bypasses monomeric intermediates during folding to retain cooperativity and avoid amyloid assembly

Polyphenols in combination with β-cyclodextrin can inhibit and disaggregate α-synuclein amyloids under cell mimicking conditions: A promising therapeutic alternative

Antifungal activities of selected essential oils against Fusarium oxysporum f. sp. lycopersici 1322, with emphasis on Syzygium aromaticum essential oil

Clues for divergent, polymorphic amyloidogenesis through dissection of amyloid forming steps of bovine carbonic anhydrase and its critical amyloid forming stretch

Structural and functional insights into an archaeal l‐asparaginase obtained through the linker‐less assembly of constituent domains. Corrigendum

Stable self-assembled nanostructured hen egg white lysozyme exhibits strong anti-proliferative activity against breast cancer cells

Curcumin binds to the pre-fibrillar aggregates of Cu/Zn superoxide dismutase (SOD1) and alters its amyloidogenic pathway resulting in reduced cytotoxicity

Domains of Pyrococcus furiosus l-asparaginase fold sequentially and assemble through strong intersubunit associative forces

Structural and functional insights into an archaeal L‐asparaginase obtained through the linker‐less assembly of constituent domains

Thermodynamic Stabilization of the Folded Domain of Prion Protein Inhibits Prion Infection in Vivo

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