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Soluble catechol-O-methyltransferase (S-COMT) is an important target protein due to its involvement in some human disorders. The main scope of the work was to develop an approach to purify and identify S-COMT. Its isoelectric point was 5.0 determined with Offgel. A simplified process including extraction, selective precipitation, ammonium sulfate precipitation, gel filtration chromatography and anion...
The mechanisms of microgravity effect on the nervous system are largely unknown, but understanding such mechanism becomes increasingly important as space exploration continues and mission duration increases. Rat tail suspension experiment is a common method to simulate the microgravity effect. In this study, the tail suspension model was established firstly. Then the O18-labeling quantitative proteomic...
In this paper, immobilized artificial membrane (IAM) was selected as the model biomembrane, and packed into capillary column as stationary phase of liquid chromatography to screen the proteins having strong interaction with biomembrane rapidly. The affinity between IAM and ten common proteins (pepsin, bovine serum albumin, casein, transferring, myosin, insulin, hemoglobin, cytochrome C, trypsin, lysozyme),...
This paper described a novel method to immobilize trypsin on IAM. The immobilization procedures involved the following steps: IAM was packed into the fused silica capillary (400 mum I.D.times690 mum O.D.times5 cm length); subsequently, trypsin was dynamically immobilized on IAM by injecting 1 mg/ml trypsin aqueous solution using the frontal chromatography. The activity of trypsin-micro-IMERs was on-line...
Monoamine oxidase (MAO) immobilized in the immobilized artificial membrane (IAM) was packed into a capillary to make a chromatography column to simulate the MAO function of catalyzing the oxidative deamination of amine in organism. MAO marked with quantum dots (QDs) was used as probe to demonstrated that MAO was immobilized in the IAM hydrophobic interface, and the enzymatic activity analysis proved...
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