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Soluble catechol-O-methyltransferase (S-COMT) is an important target protein due to its involvement in some human disorders. The main scope of the work was to develop an approach to purify and identify S-COMT. Its isoelectric point was 5.0 determined with Offgel. A simplified process including extraction, selective precipitation, ammonium sulfate precipitation, gel filtration chromatography and anion...
A method was used to investigated S-Catechol-O-Methyltransferase activity(COMT) with 3,4-dihydroxybenzoicacid as the substrate C18 column (150 mm ×4.6 mm) at 25°C. The mobile phase consisted of methanol and 0.07 % acetic acid aqueous solution with a flow rate of 1.0 mL/min at 254 nm. The optimal pH value was 8 and temperature was 45°C for S-COMT specific activity.
A high-performance liquid chromatography with electrochemical detection (HPLC-ECD) method was developed to determine the activity of S-catechol-O-methyltransferase (COMT) in rat tissues including the liver, kidney, lung ,spleen and brain. The 3,4-dihydroxybenzoic acid was used as the substrate for S-COMT in rat tissues. After incubated with different tissues, the product 4-hydroxy-3-methoxybenzoic...
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