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A sensitive and specific monoclonal antibody (Mab) against aflatoxin M 1 (AFM 1 ), named as 2C9, was selected by semi-solid HAT medium. It exhibited high affinity for AFM 1 of 1.74×10 9 L/mol and no cross-reactivity to aflatoxin B 1 , B 2 , G 1 and G 2 . Based on the antibody, an ultra-sensitive competitive enzyme-linked immunosorbent...
Three class-specific monoclonal antibodies against aflatoxins were screened by a designed strategy in which aflatoxin G 2 was used as competitor in the screening ELISA system. With a high cross-reactivity (65%) to aflatoxin G 2 , antibody 10C9 had the most similar sensitivity for five aflatoxins (AFB 1 , AFB 2 , AFG 1 , AFG 2 and AFM 1 ), whose...
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