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The article is about induction of apoptosis in human hepatoma HepG2 cells by myricetin (MYR). The human hepatoma HepG2 cells was treated with different doses myricetin for 72 h. Cytotoxicity was evaluated by SRB assay, apoptosis-related alterations in morphology ascertained by cytochemical staining(Hoechst 33258) and transmission electron microscopy (TEM). The cell cycle and apoptotic rate were quantified...
To study the effect of saponins of asparagus on apoptosis and the variations of Caspase-8, 9, 3 activity in the process of asparagus induced apoptosis in HepG-2. Cell was administrated with different concentrations of asparagus at different time, the apoptosis rate was determined by FCM with AV-PI staining, the morphology was observed by electron microscopy, caspase-8,9,3 activities was measured by...
To study the effect of growth inhibition and induction of apoptosis in sarcoma 180 cells by juglone in vivo. S180 tumor-bearing mice were set up to investigate the effects of juglone on tumor growth inhibition and apoptosis. After HE staining, the pathomorphologic changes of tumor cells were observed by optical microscope. The ultrastructural changes of tumor cells were observed by transmission electron...
Tumstatin anti-tumor peptide of 19peptide can inhibit the proliferation of melanoma tumor cell. To study its effect on the proliferation and apoptosis of different cells and verify its no anti-tumor specificity, the base sequence of 19peptide was designed and constructed engineering bacteria. The soluble 19peptide was obtained from one step chitin affinity chromatograph. By such experiment as MTT...
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