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The purpose of this work was to develop a gene delivery system that expressed acetylcholinesterase (AChE) for prolonged periods. An adeno-associated virus (AAV) expressing human AChE was constructed by cotransfecting three plasmids into HEK 293T cells. The purified vector expressed 0.17μg AChE per 1 million viral particles in culture medium in 23h, or 0.8U/ml. The AAV/hAChE was injected into muscle...
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