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Background: Given the vital role of NAD + in cell metabolism, the enzymes involved in bacterialde novo NAD + biosynthesis are possible targets for drug design against pathogenic bacteria. The first reaction in the pathway is catalysed byL-aspartate oxidase (LASPO), a flavoenzyme that converts aspartate to iminoaspartate using either molecular oxygen or fumarate as electron acceptors...
Background: Lignin degradation leads to the formation of a broad spectrum of aromatic molecules that can be used by various fungal micro-organisms as their sole source of carbon. When grown on phenolic compounds, Penicillium simplicissimum induces the strong expression of a flavin-containing vanillyl-alcohol oxidase (VAO). The enzyme catalyses the oxidation of a vast array of substrates, ranging from...
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