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The T 1 -accordion COSY experiment has been applied to acyl carrier protein (ACP) to locate the divalent ion binding sites in the protein using the paramagnetic ion, Mn 2+ , as a substitute for Ca 2+ . Replacement with Mn 2+ leads to an enhancement of proton spin-lattice (T 1 ) relaxation rates. These enhancements have a l/r 6 , distance...
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