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Background
Cryopreservation can expand the usefulness of spermatogonial stem cells (SSCs) in various fields. However, previous investigations that have attempted to modulate cryoinjury‐induced mechanisms to increase cryoprotective efficiency have mainly focused on apoptosis and necrosis.
Objectives
This study aimed to establish an effective molecular‐based cryoprotectant for SSC cryopreservation...
To study the influence of sugars and establish a serum-free freezing method for the cryopreservation of spermatogonial stem cells (SSCs).Animal study.University laboratory.C57BL/6-TgEGFP, C57BL/6 mice.Germ cells enriched from testis cells were frozen using standard freezing medium containing sugars, including monosaccharides, disaccharides, and trisaccharides at 50, 100, and 200 mM, respectively....
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