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Delayed infectivity panning (DIP) is a novel approach for the in vivo isolation of interacting protein pairs. DIP combines phage display and cell surface display of polypeptides as follows: an antigen is displayed in many copies on the surface of F+Escherichia coli cells by fusing it to a Lpp-OmpA′ hybrid. To prevent premature, non-specific infection by phage, the cells are rendered functionally F...
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