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In this work, we provide evidence for the interactions between VirB8 and VirB10, two core components of the type IV secretion system (T4SS). Using nuclear magnetic resonance experiments, we identified residues on the β1‐strand of Brucella VirB8 that undergo chemical shift changes in the presence of VirB10. Bacterial two‐hybrid experiments confirm the importance of the β1‐strand, whereas phage display...
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