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The Thermus thermophilus 3‐isopropylmalate dehydrogenase (Tt‐IPMDH) enzyme catalyses the penultimate step of the leucine‐biosynthesis pathway. It converts (2R,3S)‐3‐isopropylmalate to (2S)‐2‐isopropyl‐3‐oxosuccinate in the presence of divalent Mg2+ or Mn2+ and with the help of NAD+. In order to elucidate the detailed structural and functional mode of the enzymatic reaction, crystals of Tt‐IPMDH were...
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