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In rabbit tissues, hydroxysteroid dehydrogenase belonging to the aldo–keto reductase (AKR) superfamily exists in six isoforms (AKRs: 1C5 and 1C29–1C33), sharing >73% amino acid sequence identity. AKR1C33 is strictly NADPH-specific, in contrast to dual NADPH/NADH specificity of the other isoforms. All coenzyme-binding residues of the structurally elucidated AKR1C5 are conserved in other isoforms,...
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