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HIV‐1 antibodies targeting the carboxy‐terminal area of the membrane‐proximal external region (ctMPER) are close to exerting viral pan‐neutralization. Here, we reconstituted the ctMPER epitope as the N‐terminal extremity of the Env glycoprotein transmembrane domain helix and immobilized it onto biosensor‐supported lipid bilayers. We assessed the binding mechanism of anti‐MPER antibody 10E8 through...