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Reversible posttranslational fluorescence labeling opens exciting possibilities for advanced and superresolution microscopy techniques. In their Research Article (e202219050) Michael Holtmannspötter, Jacob Piehler et al. have engineered HaloTag variants with restored dehalogenase activity, which enzymatically exchange fluorogenic substrates at the second‐to‐minutes time scale. Thus, long‐term time‐lapse...
Selbstmarkierende Enzyme (SME) wie der HaloTag haben sich als leistungsstarke Werkzeuge in der hoch‐ und superauflösenden Fluoreszenzmikroskopie erwiesen. Neu entwickelte fluorogene SME‐Substrate ermöglichen die Bildgebung in Gegenwart von überschüssigem Farbstoff. Um diese Eigenschaft für die reversible Markierung zu nutzen, haben wir zwei Varianten von HaloTag7 mit wiederhergestellter Dehalogenase‐Aktivität...
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