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Stereoisomers of di- and tri-peptides were separated on C 18 columns after their pre-column derivatization with the chiral fluorescence labeling reagent,R (-)-4-(N,N-dimethylaminosulfonyl)-7-(3-isothiocyanatopyrrolidin-1-yl)-2,1,3- benzoxadiazole (R(-)-DBD-PyNCS). The labeling reaction was usually carried out with 1 μg of peptide sample dissolved in water in basic solution (0.1% (v/v)...
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