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The sensitivity to auxin of the H + -ATPase-mediated proton translocation was investigated in vitro using purified plasma membrane vesicles from tobacco leaves. In vivo pretreatment of tobacco plants with auxin promotes a 100-fold increase of the in vitro sensitivity to auxin. This effect is specific for biologically active auxins and is dose- and time-dependent. In addition, pretreatment...
In order to investigate the effect of the rol A gene product on the plasma membrane response to auxin, a clone of rol A-transformed tobacco was prepared. Auxin sensitivity was measured by the auxin concentration which induced the highest stimulation of H + -ATPase-mediated proton translocation on isolated plasma membrane vesicles. Both transformed and control plants exhibited identical auxin-sensitivity...
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