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Most studies use analytical grade enzymes (β-galactosidase, etc) under free-state for enzymatic conversion of lactose into galacto-oligosaccharide (GOS). This paper is a summary of earlier published works [1-4] which had four-fold objectives; i) use of an industrial grade β-galactosidase and, ii) use of a membrane as a support for enzyme immobilization, iii) use of smart polymers for enzyme bioconjugation,...
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