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The p.Arg132His mutation of isocitrate dehydrogenase 1 (IDH1R132H) is a frequent alteration and a major prognostic marker in gliomas. However, direct sequencing of highly contaminated tumor samples may fail to detect this mutation. Our objective was to evaluated the sensitivity of a newly described amplification method, coamplification at lower temperature‐PCR (COLD PCR), combined with high‐resolution...
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