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An analytical method using on-line high performance liquid chromatography-tandem mass spectrometry with electrospray ionization was developed and applied for the quantification of bis(7)-tacrine (B7T) in rat blood. B7T and pimozide (internal standard, IS) were extracted in a single step from 100μl of alkalized blood with ethyl acetate. Analytes were separated using an Extend C-18 column at 25°C. The...
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