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Adenoviruses are human pathogens increasingly used as gene therapy and vaccination vectors. However, their impact on cell metabolism is poorly characterized. We performed carbon labeling experiments with [1,2‐13C]glucose or [U‐13C]glutamine to evaluate metabolic alterations in the amniocyte‐derived, E1‐transformed 1G3 cell line during production of a human adenovirus type 5 vector (AdV5). Nonstationary...
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