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Fluorescence is a physical phenomenon described for the first time in 1852 by the British scientist George G. Stokes, famous for his work in mathematics and hydrodynamics. He observed the light emitted by a mineral after excitation (absorption of light by the mineral) by UV light. He then formulated what has become known as Stokes’ law, which says that the wavelength of fluorescence emission is longer...
Fluorescence enhanced diffuse optical tomography is an emergent diagnosis tool for the localization and the quantification of fluorescent probes. This technique can be considered as a complement but sometimes could also replace the classical ionizing radiation imaging techniques, and in particular if a simple, inexpensive, non invasive and accurate instrumentation is sought. The term molecular imaging...
This paper presents a method based on fluorescence diffuse optical tomography for reconstructing the fluorescence yield of heterogeneous and arbitrary shaped medium such as small animals. The experimental set-up is presented and the associated reconstruction method making mouse inspection without immersion in optical index matching liquid (Intralipid and ink) possible is detailed. Some phantom experiments...
This paper presents a new fluorescence diffuse optical tomographic (fDOT) system and its associated reconstruction method. It is able to reconstruct the fluorescence yield even in heterogeneous and highly attenuating regions. Furthermore our reconstruction method makes mouse inspection without immersion in optical index matching liquid (Intralipid and ink) possible. Some phantom experiments have been...
A fluorescence enhanced diffuse optical tomography algorithm taking into account the optical heterogeneities is presented. It is suitable for continuous wave acquisitions and naturally handles complex shaped object. Therefore, it allows in vivo studies of small animals injected with target-specific fluorescent probes without immersion fluid. A comparison with previous methods conducted on simulations...
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