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To improve differential diagnosis of Bacillus cereus group and minimize a risk of bacilli related disease, a multiplex quantitative real-time PCR assay for a one step detection and differentiation of B. cereus sensu lato and emetic B. cereus spores in milk was developed.A qPCR assay with primers and probe targeting gyrB (gyrase B) gene was successfully established and applied in a multiplex qPCR approach...
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