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Various high-mannose-type glycan modifications of dihydrofolate reductase (DHFR) were achieved by ligand-based approach using glycan–methotrexate (MTX) conjugates as tight binding glycan bearing ligands for DHFR. The resulting glycan–MTX conjugates and the corresponding artificial glycoproteins could be useful as oligosaccharide- and glycoprotein-probes to perform quantitative analysis of glycan recognizing...
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