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Trypsin, as one of important proteases, is specific for catalyzing the hydrolysis of peptide and ester bonds containing lysine and arginine residues at the C-terminus. The level of trypsin in biological fluids can serve as a reliable and specific diagnostic biomarker for pancreatic function and its pathological changes. Herein, we demonstrate the application of phosphorescent Cu NCs for trypsin detection...
High levels of triacylglycerols (TGs) have been linked to cardiovascular disease and liver diseases. Comprehensively analyzing TGs is helpful to understand the TGs functions in these diseases. However, due to the existence of a large number of isomers TGs and the lack of commercial standards, precise analysis of individual triacylglycerol (TG) with specific fatty acid chain composition is full of...
A novel anthrancene-dopamine thioether L compound was designed as fluorescent probe for detecting Hg2+ in living cells sample. L exhibits a good sensitive and selective recognition towards Hg2+ ions in the presence of other important relevant metal ions and amino acids in HEPES solution. The addition of Hg2+ causes a marked enhancement in the fluorescence emission intensity with the detection limit...
Ovarian cancer (OC) is the most common cause of death from gynecologic malignancies in women. The identification of reliable diagnostic biomarkers for the early detection of this deadly disease is critical for reducing the mortality rate of OC. Plasma lysophosphatidic acid (LPA) levels were increased from OC patients vs. healthy controls. Therefore, lipidomics may represent an excellent developing...
A carbon paste electrode modified with tin was used for the determination of trace cadmium by anodic stripping voltammetry. The electroanalytical performance for the determination of Cd(II) on the tin-coated carbon paste electrode(SnF-CPE) was better than that on the carbon paste electrode. The measuring conditions have been optimized. The measurement of trace cadmium on the SnF-CPE has the best response...
A class of novel heterocyclic compounds 1a–1c has been designed and synthesized. And our initial discovery is that these compounds can effectively recognize HSO 4− . With the addition of HSO 4− , a new energy band appears at 360–390nm in the absorption spectra. The emission spectrum (λ ex =328 nm) undergoes an important change in its fluorescent effect in the presence...
Screening G-quadruplex ligands from natural plants is important because the ligands may be potential antitumor drugs. A new screening strategy is proposed based on the combination of dialysis and G-quadruplex recognition technique which could separates G-quadruplex ligand from natural extracts and elucidate the structure of this ligand. This result offers a novel approach to obtain active antitumor...
A couple of new fluorescent probes, based on diethoxycarbonyl glycoluril, were synthesized and characterized by 1 H NMR, X-ray crystal structure. The probes were found to be relatively highly fluorescence with quantum yields of 0.20 and 0.18, respectively, at room temperature in THF solution. Both of the proposed probes were tested using different phenolic compounds, showing very high selectivity...
Linear solvation energy relationships (LSERs) have been recognized as a useful model for investigating the chemical forces behind the partition coefficients between vapor molecules and absorbents. This study is the first to determine the solvation properties of monolayer-protected gold nanoclusters (MPCs) with different surface ligands. The ratio of partition coefficients/MPC density (K/ρ) of 18 volatile...
In this paper, 3.5-generation polyamidoamine dendrimers (3.5G-D)-porphyrin (P) dual luminescence molecule (3.5G-D-P) was developed as a new phosphorescence-labeling reagent. Meanwhile, the room temperature phosphorescence (RTP) characteristics of 3.5G-D-P and its product of labeling triticum vulgaris lectin (WGA) on the surface of polyamide membrane (PAM) were studied. Results showed that in the presence...
2,2,6,6-Tetramethyl-4-hydroxypiperidine-1-oxyl (TMHPO) shows catalytic electroactivity in homogeneous oxidation of benzoyl hydrazine (BH) on a glassy carbon electrode. The catalytic current is affected by the concentration of TMHPO and the pH of the solution. A possible catalytic reaction mechanism is proposed and a non-toxic, convenient method for the determination of BH in the concentration range...
A new type of magnetic polymer microspheres containing ketone groups on the surface was synthesized. It can be reacted with hydrazine to produce electroactive adduct. Reduction of this derivative that was aggregated on the magnetic electrode is possible and effective in indirect determination of hydrazine. The experimental conditions and electrode structure were discussed. Under optimum conditions,...
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