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Proteome display allows the functional display of positive selected open reading frames (ORF) of randomly fragmented whole genomes or even metagenomes, thus covering all possible proteins encoded by a pathogen or an entire microbiome. Immunogenic proteins can then be selected from these libraries using patient-derived immunoglobulins (serum). Hence, proteome display allows the identification of immunogenic...
Intrabodies are recombinant antibody fragments produced inside of a living cell in a way to bind their antigen in that same cell, to interfere with its function. As they strictly work on the protein level, intrabodies allow to bypass disadvantages of gene-targeted knock-out animals and knockdown techniques (RNAi, miRNA and small molecule inhibitors). Recently, a regulated knock-down strength was illustrated...
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