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Skull optical clearing window permits us to perform in vivo cortical imaging without craniotomy, but mainly limits to visible (vis)‐near infrared (NIR)‐I light imaging. If the skull optical clearing window is available for NIR‐II, the imaging depth will be further enhanced. Herein, we developed a vis‐NIR‐II skull optical clearing agents with deuterium oxide instead of water, which could make the skull...
Optical clearing skull window is a novel technique compared to the traditional surgery based cranial window, which could reduce the scattering of skull and make it transparent to visible light. Here, the NIR‐II optimized skull clearing window was established. In the application of NIR‐II excited THG microscopy, the imaging depth was more than 3 times as it without optical clearing, and close to it...
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