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The reaction of hydroxyl radicals with γ-glutamyl-methionine and γ-glutamyl-glycyl-methionyl-glycine at neutral pH results in similar N-terminal decarboxylation efficiency. The underlying mechanism involves an intramolecular proton transfer from the protonated N-terminal amino group of the glutamyl moiety to an initially formed hydroxy sulphuranyl radical at the methionine residue. This process...
The SR Ca-ATPase in skeletal muscle SR vesicles isolated from young adult (5 months) and aged (28 months) rats was analyzed for nitrotyrosine. Only the SERCA2a isoform contained significant amounts with approximately one and four nitrotyrosine residues per young and old Ca-ATPase, respectively. The in vitro exposure of SR vesicles of young rats to peroxynitrite yielded selective nitration of the...
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