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An anti-inflammatory peptide was identified in lupine protein hydrolysate (LPH) obtained by enzymatic hydrolysis of lupine protein isolate (LPI) with Alcalase. LPH was separated in two fractions by ultrafiltration, and anti-inflammatory activity was evaluated by analysis of the expression of different cytokines in THP-1-derived macrophages by quantitative PCR (qPCR). Fraction <10 kDa was purified...
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