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Novel hydrophobic adsorbents were synthesized by immobilizing butyl derivative onto the highly cross-linked agarose beads manufactured in China, which are used as matrix. The effects of the spacer arm length (3C, 8C, and 10C) and ligand density (from 13 to 45 μmol/mL) on the hydrophobicity were investigated using purified Hepatitis B surface antigen (HBsAg) expressed by CHO cell lines. Also, considering...
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