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Using recombinant human tau protein phosphorylated by a brain extract and the glycogen synthase kinase-3β in the absence or the presence of heparin, we showed that phosphorylation-dependent antibody AD2 recognition only requires phosphorylated Ser-396. By the Spot multiple peptide synthesis method, we showed that Tyr-394, Ser P -396 and Pro-397 are critical for AD2 binding. A decrease in the...
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