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The promotion effect of titania nanoparticles (nano-TiO 2 ) on the direct electron transfer between lactate dehydrogenase (LDH) and the silica sol–gel modified gold electrode was investigated by adding nano-TiO 2 (50nm) in the modification process. This nano-TiO 2 –LDH electrode showed a pair of quasi-reversible cyclic voltammetry peaks with the formal potential of 70mV (vs...
In this paper, differential-pulse voltammetry (DPV) was applied to study the effects of aluminum (Al(III)) on the lactate dehydrogenase (LDH) activity, KmNADH and ν max in the enzyme promoting catalytic reaction of “Pyruvate+NADH+H+⇄LDHLactate+NAD+” by monitoring DPV reduction current of NAD + . The changes of Al's influence on the LDH activities caused by the concentration of LDH,...
The existence of dimethylselenium (DMSe) and dimethyldiselenium (DMDSe) in some environmental samples can cause serious interference on Se(IV) determination by hydride generation atomic fluorescence spectrometry (HG-AFS) due to their contribution on HG-response. A flow injection separation and preconcentration system coupled to HG-AFS was therefore developed by on-line coprecipitation in a knotted...
In weakly acidic buffer medium, the interaction of amikacin with calf thymus DNA, yeast RNA and denatured DNA has been investigated by using resonance Rayleigh scattering (RRS) technique. The result shows that calf thymus DNA is capable of enhancing the RRS intensity of the amikacin, while yeast RNA and denatured DNA have very little enhancement effect. Based on the characteristics, a sensitive assay...
The electrochemical behavior of maltol on a glassy carbon (GC) electrode was investigated. The results were applied to differential pulse voltammetric determination of maltol in beverages pretreated by ultrafiltration. Under the optimum experimental conditions, the linear range is 1×10 −5 to 6×10 −4 moll −1 maltol and the relative standard deviation for 0.4mmoll −1 ...
In the dilute sulfuric acid medium, the reactions of proteins with some heteropoly compounds (HPC) including some heteropoly acids (HPA) and their reducing products would result in the enhancement of resonance Rayleigh scattering (RRS) intensity and the appearance of the corresponding RRS spectra. Their maximum scattering peaks were all at 470nm, and there were four smaller peaks at 400, 510, 800...
The determination of trace levels of aluminum by high performance liquid chromatography (HPLC) with spectrophotometric detection using quercetin, a bioactive substance as a pre-column reagent, is developed in this paper. The Al–quercetin chelate was separated on a reversed-phase ODS column with a mobile phase consisting of 70% water (pH 1.0 with perchloric acid) and 30% methanol, and detected at its...
The determination of trace levels of aluminum in natural waters with rubeanic acid (RA) by adsorption chronopotentiometry is developed in this paper. Optimum experimental conditions include an accumulation potential of -0.40 V, accumulation time of 60 s, and a RA concentration of 6x10 -6 M in 0.2 M NaAc-HAc buffer solution (pH 4.6). The response is linear over the 1x10 -8 ...
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