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Objective To clarify the molecular mechanism of GDP-l-fucose biosynthesis in Mortierella alpina. Results Analysis of the M. alpina genome suggests that there were two isofunctional GDP-d-mannose-4,6-dehydratase genes (GMD1 and GMD2) that have never been found in a microorganism before. GMD2 was expressed heterologously in Escherichia coli and purified to homogeneity. The addition of exogenous NAD...
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