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Mass transfer effects were investigated for the synthesis of ampicillin and amoxicillin, at pH 6.5 and 25 °C, catalyzed by penicillin G acylase immobilized on agarose. The influence of external mass transfer was analysed using different stirring rates, ranging form 200 to 800 rpm. Above 400 rpm, the film resistance may be neglected. Intra-particle diffusion limitation was investigated using biocatalysts...
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