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The gene product Sll1127 is a predicted 1,4-dihydroxy-2-naphthoyl-CoA synthase catalyzing an intramolecular Claisen condensation in the phylloquinone biosynthesis of the cyanobacterium Synechocystis sp. PCC 6803. This predicted catalytic function has been verified and the enzyme has been characterized for the first time with kcat= 0.013 s−1 and KM = 9 μM. Its catalytic activity is found to strictly...
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