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2,2'-Dithiobis(5-nitropyridine) has proven to be a suitable reagent for the activation of the thiol function of cysteine in peptides and subsequent asymmetric disulfide formation. The utility of this reagent is tested in the preparation of de novo designed cytochrome model heterodimeric 62-mer peptides.
N α -Boc-N α -methyl-N ω,ω′ -bis(benzyloxycarbonyl)-L-arginine and N α -Boc-N α -methyl-N δ -benzyloxycarbonyl-L-ornithine have been synthesized starting with Boc-L-Gln. The γ-carboxamide of Boc-L-Gln was dehydrated to a nitrile group and the resulting compound is selectively methylated providing N-Boc-2-methylamino-4-cyanobutyric acid. The nitrile is...
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