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The advantages of using off-resonance rf fields in heteronuclear self-relaxation experiments are explored on a fully 15N-enriched protein. It is firstly shown that in the absence of slow motions the longitudinal and transverse 15N self-relaxation rate values derived with this method are in agreement with the ones measured by the classical inversion-recovery and Carr–Purcell–Meiboom–Gill (CPMG) sequences,...
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