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The wide-ranging effects of RirA, a novel Fe-responsive regulator of gene expression in Rhizobium leguminosarum bv. viciae, were monitored on 2D gels. Approximately 100 proteins were expressed at higher levels in a RirA− mutant, compared to wild type. These included the products of the sufS2BCDS1XA operon, which probably specifies the synthesis of [FeS] clusters. Using lac fusions, this operon...
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