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Simultaneous measurement of redox potential (Eh) and determination of H2 evolution kinetics using a pair of titanium silicate and platinum redox electrodes in fermenting cultures of Escherichia coli wild type and different mutants lacking hydrogenases 1 (Hyd‐1) or 2 (Hyd‐2) revealed that Hyd‐1 controls the onset of H2 evolution at slightly alkaline pH (pH 7.5) and under oxidizing Eh. In addition,...
The anaerobically inducible l-serine dehydratase, TdcG, from Escherichia coli was characterized. Based on UV-visible spectroscopy, iron and labile sulfide analyses, the homodimeric enzyme is proposed to have two oxygen-labile [4Fe-4S] 2+ clusters. Anaerobically isolated dimeric TdcG had a k cat of 544 s -1 and an apparent K M for l-serine of 4.8...
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