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[NiFe]-hydrogenases are multimeric proteins. The large subunit contains the NiFe(CN) 2 CO bimetallic active center and the small subunit contains Fe-S clusters. Biosynthesis and assembly of the NiFe(CN) 2 CO active center requires six Hyp accessory proteins. The synthesis of the CN − ligands is catalyzed by the combined actions of HypF and HypE using carbamoylphosphate as a...
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