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Several variants of fast-activating, voltage-dependant K + channels exist in the nervous system where they control cell excitability and synaptic transmission, some of which are blocked selectively by α-dendrotoxin. Cloning of a K + channel from bovine genomic DNA was achieved using a primer based on the N-terminal sequence of the larger subunit from the purified toxin acceptor, in...
We have used the polymerase chain reaction (PCR) with primers against the S5 and S6 regions of voltage-gated K + channels to identify 8 different specific amplification products using poly(A) + RNA isolated from islets of Langerhans from obese hyperglycemic (ob/ob) mice and from the two insulin-producing cell lines HIT T15 and RINmSF. Sequence analysis suggests that they derive from...
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