The Infona portal uses cookies, i.e. strings of text saved by a browser on the user's device. The portal can access those files and use them to remember the user's data, such as their chosen settings (screen view, interface language, etc.), or their login data. By using the Infona portal the user accepts automatic saving and using this information for portal operation purposes. More information on the subject can be found in the Privacy Policy and Terms of Service. By closing this window the user confirms that they have read the information on cookie usage, and they accept the privacy policy and the way cookies are used by the portal. You can change the cookie settings in your browser.
We selected and investigated nine G-quadruplex (G4)-forming aptamers originally designed against different proteins involved in the regulation of cellular proliferation (STAT3, nucleolin, TOP1, SP1, VEGF, and SHP-2) and considered to be potential anticancer agents. We showed that under physiological conditions all the aptamers form stable G4s of different topology. G4 aptamers designed against STAT3,...
Systematic study of chemical reactivity of non-Watson–Crick base pairs depending on their type and microenvironment was performed on a model system that represents two sets of synthetic DNA duplexes with all types of mismatched and unmatched bases flanked by T·A or G·C pairs. Using comparative cleavage pattern analysis, we identified the main and additional target bases and performed quantitative...
Favorable photo-physical properties and high affinity to nucleic acids make new fluorescent cyanine dyes of the SYBR-type particularly useful for DNA and RNA visualization. The growing popularity of SYBR-type dyes is also explained by the fact that removal of the dye from the nucleic acids by ethanol precipitation is more efficient and less time-consuming than the phenol–chloroform extraction applied...
The expression and enzymatic activities of insecticide resistance-related cytochrome P450B are increased by the treatment with 20-hydroxyecdysone (20HE) inD. melanogasterOregon R flies. We have explored the role of this hormone in the maintenance of P450B basal expression. Arrest of ecdysone synthesis led to a decrease in CYP6A2 mRNA level, as well as in P450B expression and activities. This effect...
Set the date range to filter the displayed results. You can set a starting date, ending date or both. You can enter the dates manually or choose them from the calendar.