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Nitric oxide (NO) is synthesised by a two-step oxidation of l-arginine (l-Arg) in the active site of nitric oxide synthase (NOS) with formation of an intermediate, N ω -hydroxy-l-Arg (NOHA). Crystal structures of NOSs have shown the importance of an active-site Val567 residue (numbered for rat neuronal NOS, nNOS) interacting with non-amino acid substrates. To investigate the role of this Val...
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