Search results for: Masaharu Seno

Items from 1 to 20 out of 44 results

article

Optimization of production and characterization of a recombinant soluble human Cripto‐1 protein inhibiting self‐renewal of cancer stem cells

Said M. Afify, Ghmkin Hassan, Hend M. Nawara, Maram H Zahra, more

Journal of Cellular Biochemistry > 123 > 7 > 1183 - 1196

Human Cripto‐1 is a member of the epidermal growth factor (EGF)‐Cripto‐FRL‐1‐Cryptic (CFC) family family and performs critical roles in cancer and various pathological and developmental processes. Recently we demonstrated that a soluble form of Cripto‐1 suppresses the self‐renewal and enhances the differentiation of cancer stem cells (CSCs). A functional form of soluble Cripto‐1 was found to be difficult...

article

Diphenyleneiodonium efficiently inhibits the characteristics of a cancer stem cell model derived from induced pluripotent stem cells

Sadia Monzur, Ghmkin Hassan, Said M. Afify, Kazuki Kumon, more

Cell Biochemistry and Function > 40 > 3 > 310 - 320

Diphenyleneiodonium (DPI) has long been evaluated as an anticancer drug inhibiting NADPH oxidase, the IC₅₀ in several cancer cell lines was reported 10 µM, which is too high for efficacy. In this study, we employed miPS‐Huh7cmP cells, which we previously established as a cancer stem cell (CSC) model from induced pluripotent stem cells, to reevaluate the efficacy of DPI because CSCs are currently one...

article

Metformin suppresses self‐renewal and stemness of cancer stem cell models derived from pluripotent stem cells

Maram H. Zahra, Said M. Afify, Ghmkin Hassan, Hend M. Nawara, more

Cell Biochemistry and Function > 39 > 7 > 896 - 907

Metformin exhibits anti‐cancer activities in various types of tumours while it is prescribed as the first‐line drug for type 2 diabetes. Since new evidence has recently suggested that metformin could target cancer stem cells (CSCs) and prevent their recurrence, repositioning of metformin could be considered as a candidate for anti‐CSC agent. In this study, we assessed the effect of metformin on the...

article

An assay for cancer stem cell‐induced angiogenesis on chick chorioallantoic membrane

Hend M. Nawara, Said M. Afify, Ghmkin Hassan, Maram H. Zahra, more

Cell Biology International > 45 > 4 > 749 - 756

Angiogenesis is generally involved in tumor growth and metastasis. Cancer stem cells (CSCs) are considered to facilitate the angiogenesis. Therefore, CSCs could be the effective targets to stop angiogenesis. Recently, our group successfully generated CSC models from induced pluripotent stem cells (iPSCs) in the presence of conditioned medium derived from cancer derived cells. These novel model CSCs...

article

HSP‐enriched properties of extracellular vesicles involve survival of metastatic oral cancer cells

Kisho Ono, Takanori Eguchi, Chiharu Sogawa, Stuart K. Calderwood, more

Journal of Cellular Biochemistry > 119 > 9 > 7350 - 7362

Cancer cells often secrete extracellular vesicles (EVs) that carry heat shock proteins (HSPs) with roles in tumor progression. Oral squamous cell carcinoma (OSCC) belongs to head and neck cancers (HNC) whose lymph‐node‐metastases often lead to poor prognosis. We have examined the EV proteome of OSCC cells and found abundant secretion of HSP90‐enriched EVs in lymph‐node‐metastatic OSCC cells. Double...

article

Synthesis and assembly of Hepatitis B virus envelope protein-derived particles in Escherichia coli

Hao Li, Keisuke Onbe, Qiushi Liu, Masumi Iijima, more

Biochemical and Biophysical Research Communications > 2017 > 490 > 2 > 155-160

Hepatitis B virus (HBV) envelope particles have been synthesized in eukaryotic cells (e.g., mammalian cells, insect cells, and yeast cells) as an HB vaccine immunogen and drug delivery system (DDS) nanocarrier. Many researchers had made attempts to synthesize the particles in Escherichia coli for minimize the cost and time for producing HBV envelope particles, but the protein was too deleterious to...

article

Anticancer effects of novel thalidomide analogs in A549 cells through inhibition of vascular endothelial growth factor and matrix metalloproteinase-2

Bishoy El-Aarag, Tomonari Kasai, Junko Masuda, Hussein Agwa, more

Biomedicine & Pharmacotherapy > 2017 > 85 > C > 549-555

Lung cancer is one of the major causes of cancer-related mortality worldwide, and non-small-cell lung cancer is the most common form of lung cancer. Several studies had shown that thalidomide has potential for prevention and therapy of cancer. Therefore, the current study aimed to investigate the antitumor effects of two novel thalidomide analogs in human lung cancer A549 cells. The antiproliferative,...

article

Transient Tcf3 Gene Repression by TALE-Transcription Factor Targeting

Junko Masuda, Hiroshi Kawamoto, Warren Strober, Eiji Takayama, more

Applied Biochemistry and Biotechnology > 2016 > 180 > 8 > 1559-1573

Transplantation of hematopoietic stem and progenitor cells (HSCs) i.e., self-renewing cells that retain multipotentiality, is now a widely performed therapy for many hematopoietic diseases. However, these cells are present in low number and are subject to replicative senescence after extraction; thus, the acquisition of sufficient numbers of cells for transplantation requires donors able to provide...

article

Synthesis and in vitro cancer cell growth inhibition evaluation of 11-amino-modified 5-Me-indolo[2,3-b]quinolines and their COMPARE analyses

Masashi Okada, Zhen-Wu Mei, Md. Imran Hossain, Li Wang, more

Medicinal Chemistry Research > 2016 > 25 > 5 > 879-892

A plant-derived neocryptolepine core, the 5-Me-indolo[2,3-b]quinoline skeleton, was emblazoned with substituents at C11 and C2 and then tested against various cancer cell lines to find potent anticancer agents. In the in vitro antiproliferative activity assay against the breast cancer MDA-MB-453 cell line, the attachment of alkylamino substituents at C11 of the 5-Me-indolo[2,3-b]quinoline induced...

article

In vitro anti-proliferative and anti-angiogenic activities of thalidomide dithiocarbamate analogs

Bishoy Y.A. El-Aarag, Tomonari Kasai, Magdy A.H. Zahran, Nadia I. Zakhary, more

International Immunopharmacology > 2014 > 21 > 2 > 283-292

Inhibition of angiogenesis is currently perceived as a promising strategy in the treatment of cancer. The anti-angiogenicity of thalidomide has inspired a second wave of research on this teratogenic drug. The present study aimed to investigate the anti-proliferative and anti-angiogenic activities of two thalidomide dithiocarbamate analogs by studying their anti-proliferative effects on human umbilical...

article

Cancer stem cells maintain a hierarchy of differentiation by creating their niche

Shuichi Matsuda, Ting Yan, Akifumi Mizutani, Tatsuyuki Sota, more

International Journal of Cancer > 135 > 1 > 27 - 36

The self‐renewal and differentiation properties of cancer stem cells (CSCs) are regulated and maintained by the CSC niche. However, the mechanism of this maintenance, especially the maintenance contributed by differentiated cancer cells, remains to be fully elucidated. Recently, we have established a model of CSCs, miPS‐LLCcm, from mouse induced pluripotent stem cells (miPSCs). In vitro cultured miPS‐LLCcm...

article

Steering Target Selectivity and Potency by Fragment‐Based De Novo Drug Design

Tiago Rodrigues, Takayuki Kudoh, Filip Roudnicky, Yi Fan Lim, more

Angewandte Chemie > 125 > 38 > 10190 - 10193

Kinase‐Inhibitoren: Liganden‐basiertes De‐novo‐Design wurde als geeignete Technik für die schnelle Erzeugung neuer Verbindungen mit einem gewünschten biochemischen Profil bestätigt. Die Studie führte zur Entdeckung des derzeitig selektivsten Hemmers (rechts im Schema) der VEGFR‐2‐Kinase (VEGFR=vascular endothelial growth factor receptor) und bietet so einen ausgezeichneten Ausgangspunkt für die Wirkstoff‐Entwicklung...

article

Steering Target Selectivity and Potency by Fragment‐Based De Novo Drug Design

Tiago Rodrigues, Takayuki Kudoh, Filip Roudnicky, Yi Fan Lim, more

Angewandte Chemie International Edition > 52 > 38 > 10006 - 10009

Kinase inhibitors: Ligand‐based de novo design is validated as a viable technology for rapidly generating innovative compounds possessing the desired biochemical profile. The study discloses the discovery of the most selective vascular endothelial growth factor receptor‐2 (VEGFR‐2) kinase inhibitor (right in scheme) known to date as prime lead for antiangiogenic drug development.

article

Cripto-1 enhances the canonical Wnt/β-catenin signaling pathway by binding to LRP5 and LRP6 co-receptors

Tadahiro Nagaoka, Hideaki Karasawa, Thomas Turbyville, Maria-Cristina Rangel, more

Cellular Signalling > 2013 > 25 > 1 > 178-189

Cripto-1 is implicated in multiple cellular events, including cell proliferation, motility and angiogenesis, through the activation of an intricate network of signaling pathways. A crosstalk between Cripto-1 and the canonical Wnt/β-catenin signaling pathway has been previously described. In fact, Cripto-1 is a downstream target gene of the canonical Wnt/β-catenin signaling pathway in the embryo and...

article

Eosinophil cationic protein enhances stabilization of β-catenin during cardiomyocyte differentiation in P19CL6 embryonal carcinoma cells

Guoliang Jin, Akifumi Mizutani, Takayuki Fukuda, Takayuki Otani, more

Molecular Biology Reports > 2013 > 40 > 4 > 3165-3171

Prior to gastrulation, the Wnt signaling pathway through stabilized β-catenin enhances the differentiation of mouse ES cell into cardiomyocytes. We have recently shown that cardiomyocyte differentiation is enhanced by eosinophil cationic protein (ECP) through accelerated expression of marker genes of early cardiac differentiation. Furthermore, ECP enhanced the expression of Wnt3a in P19CL6 cells which...

article

Extracellular matrix modulates insulin production during differentiation of AR42J cells: Functional role of Pax6 transcription factor

Kohei Hamamoto, Satoko Yamada, Akemi Hara, Tsutomu Kodera, more

Journal of Cellular Biochemistry > 112 > 1 > 318 - 329

Extracellular matrix (ECM) modulates differentiation of pancreatic β‐cells during development. However, the mechanism by which ECM proteins modulate differentiation is not totally clear. We investigated the effect of ECM proteins on differentiation β‐cells in vitro. We investigated the effect of basement membrane ECM on differentiation of AR42J cells and rat ductal cells. First, we examined the effect...

article

Production of biologically active IgG hinge-tag soluble epidermal growth factor receptors (ErbB)

Takayuki Otani, Toshihiro Hashizume, Tadahiro Nagaoka, Tomoko Fukuda, more

Biotechnology Letters > 2010 > 32 > 3 > 361-366

The extracellular domains (ECD) of epidermal growth factor receptors, ErbB1, 2, 3 and 4, were designed as soluble dimeric forms. Each ECD was fused to a short hinge region derived from IgG, such that the stable dimer could be formed with disulfide bridges. This hinge-tagged design minimized the molecular weight to approximately 50% of the conventional Fc-fusion design without an Fc domain of IgG....

article

Delivery of sodium borocaptate to glioma cells using immunoliposome conjugated with anti-EGFR antibodies by ZZ-His

Bin Feng, Kazuhito Tomizawa, Hiroyuki Michiue, Shin-ichi Miyatake, more

Biomaterials > 2009 > 30 > 9 > 1746-1755

Nanoparticles are effective of delivering cargo into cells. Here, sodium borocaptate (BSH) was encapsulated in liposomes composed of nickel lipid, and anti-epidermal growth factor receptor (EGFR) antibodies were conjugated to the liposomes using the antibody affinity motif of protein A (ZZ) as an adaptor (immunoliposomes). The immunoliposomes were used to deliver BSH into EGFR-overexpressing glioma...

article

Identification of TM9SF2 as a candidate of the cell surface marker common to breast carcinoma cells

Samah Abou-Sharieha, Yuh Sugii, Tuoya, Dongwei Yu, more

Clinical Oncology and Cancer Research > 2009 > 6 > 1 > 1-9

Objective We aimed identification of cell surface molecules, which might serve as diagnostic biomarkers or useful targets for therapies, in breast cancer. Methods We developed unique DNA microarray coupled with spherical self-organizing map (sSOM) analysis to characterize cells and tissues by the cell surface markers. In the microarray 1,797 probes for human genes coding membrane bound proteins...

article

Spherical self-organizing map as a helpful tool to identify category-specific cell surface markers

Tuoya, Yuh Sugii, Hitomi Satoh, Dongwei Yu, more

Biochemical and Biophysical Research Communications > 2008 > 376 > 2 > 414-418

We analyzed gene expression profiles of five tumor cell lines (NB2a, NB41A3, C1300N18, BC3H1, and Neuro2a) derived from a category of nervous system using our originally developed cell surface marker DNA microarray in order to search for tumor-specific cell surface markers common to these cells. To visualize the expression patterns and to extract candidate genes of interest based on the expression...

Keywords:
ABBREVATIONS.PDB PROTEIN DATA BANK RMSD ROOT MEAN SQUARE DEVIATION H-BONDS HYDROGEN BONDS MD MOLECULAR DYNAMICS HEGF HUMAN EPIDERMAL GROWTH FACTOR HHB-EGF HUMAN HEPARIN BINDING EPIDERMAL GROWTH FACTOR HTGF HUMAN TRANSFORMING GROWTH FACTOR ALPHA HHRG-1 HUMAN HEREGULIN ONE ALPHA HHRG-1 HUMAN HEREGULIN ONE BETA HBTC HUMAN BETACELLULIN EGF EPIDERMAL GROWTH FACTOR HB-EGF HEPARIN BINDING EPIDERMAL GROWTH FACTOR TGF TRANSFORMING GROWTH FACTOR ALPHA BTC BETACELLULIN HRG HEREGULIN EP EPIREGULIN AR AMPHIREGULIN EGFR EPIDERMAL GROWTH FACTOR RECEPTOR ERBB-[1,2,3,4]

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INFONA - science communication portal

Search results for: Masaharu Seno

Optimization of production and characterization of a recombinant soluble human Cripto‐1 protein inhibiting self‐renewal of cancer stem cells

Diphenyleneiodonium efficiently inhibits the characteristics of a cancer stem cell model derived from induced pluripotent stem cells

Metformin suppresses self‐renewal and stemness of cancer stem cell models derived from pluripotent stem cells

An assay for cancer stem cell‐induced angiogenesis on chick chorioallantoic membrane

HSP‐enriched properties of extracellular vesicles involve survival of metastatic oral cancer cells

Synthesis and assembly of Hepatitis B virus envelope protein-derived particles in Escherichia coli

Anticancer effects of novel thalidomide analogs in A549 cells through inhibition of vascular endothelial growth factor and matrix metalloproteinase-2

Transient Tcf3 Gene Repression by TALE-Transcription Factor Targeting

Synthesis and in vitro cancer cell growth inhibition evaluation of 11-amino-modified 5-Me-indolo[2,3-b]quinolines and their COMPARE analyses

In vitro anti-proliferative and anti-angiogenic activities of thalidomide dithiocarbamate analogs

Cancer stem cells maintain a hierarchy of differentiation by creating their niche

Steering Target Selectivity and Potency by Fragment‐Based De Novo Drug Design

Steering Target Selectivity and Potency by Fragment‐Based De Novo Drug Design

Cripto-1 enhances the canonical Wnt/β-catenin signaling pathway by binding to LRP5 and LRP6 co-receptors

Eosinophil cationic protein enhances stabilization of β-catenin during cardiomyocyte differentiation in P19CL6 embryonal carcinoma cells

Extracellular matrix modulates insulin production during differentiation of AR42J cells: Functional role of Pax6 transcription factor

Production of biologically active IgG hinge-tag soluble epidermal growth factor receptors (ErbB)

Delivery of sodium borocaptate to glioma cells using immunoliposome conjugated with anti-EGFR antibodies by ZZ-His

Identification of TM9SF2 as a candidate of the cell surface marker common to breast carcinoma cells

Spherical self-organizing map as a helpful tool to identify category-specific cell surface markers

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