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We examined the effects of insulin on fatty acid uptake in L-cell fibroblasts, usingcis-parinaric acid to measure uptake rates in the absence of esterification and [3H]oleic acid to measure uptake rates in the presence of esterification. L-cells exhibited both high and low affinity insulin binding sites withKdof 23 nMand 220 nMand a cellular density of 1.4 and 6.8 × 105sites/cell, respectively. Insulin...
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