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When the 34kDa kinase domain of human spleen tyrosine kinase (Syk-KD) was expressed as a C-terminally His-tagged protein in baculovirus-infected Sf-21 insect cells, the purified protein included two forms that migrated slightly differently in SDS–polyacrylamide gel electrophoresis. Intact mass analysis and LC–MS/MS peptide mapping showed that the major and faster-migrating product had the intended...
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