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A method for introduction of dimethylamionapthamide and fluorescein labels as a fluorescence energy donor and acceptor pair into 2'-positions of DNA duplexes has been described. It has been shown that the attachment of these bulky fluorophores to the sugar 2'-position at the terminal fraying end of each oligonucleotide strand does not alter the normal thermal stability and global conformation of...
Oligonucleotide derivatives having a dansyl fluorescence label at the 2'-position of a ribonucleoside have been synthesized by using 5'-dimethoxytrityl 2'-(dansylamino)uridine 3'-phosphorobisdiethylamidite. The oligonucleotide containing a dansyl-modified uridine at the 5'-terminal position exhibits normal binding affinity for a complementary DNA segment in an aqueous buffer solution (pH 7.0). A significant...
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