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A surface plasmon resonance based RNA aptasensor for rapid detection of natively folded type A botulinum neurotoxin is reported. Using detoxified recombinant type A botulinum neurotoxin as the surrogate, the aptasensor detects active toxin within 90min. The detection limit of the aptasensor in phosphate buffered saline, carrot juice, and fat free milk is 5.8ng/ml, 20.3ng/ml and 23.4ng/ml, respectively,...
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