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Common metabolomics platforms require about 106 cells, which has a limited throughput due to the time‐consuming steps of cell culture and preparation. There is a demand for metabolic profiling methods to improve analytical throughput and detection sensitivity based on small amount of cells. In this study, we proposed a high‐throughput scheme, integrating 96‐well plate cell cultivation, in‐situ cell...
In this work a chip‐based nano HPLC coupled MS (HPLC‐chip/MS) method with a simple sample preparation procedure was developed for the flavonoid profiling of soybean. The analytical properties of the method including the linearity (R2, 0.992–0.995), reproducibility (RSD, 1.50–7.66%), intraday precision (RSD, 1.41–5.14%) and interday precision (RSD, 2.76–16.90%) were satisfactory. Compared with the...
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